Luminex Flock Monitor Quick Guide Manuel d'utilisateur télécharger pdf

Quick Guide

89-30000-00-261 Revision A - Page 1 of 2

Sample, Reagent and Plate Preparation

Before setting up the Flock Monitor assay plate, use the following procedures to prepare fresh reagents and sample

dilutions. Allow all components to equilibrate to room temperature, and prepare aliquots and dilutions immediately

prior to performing the assay.

• May be a LifeSep 96F hand held magnet for maual washing (see http://www.luminexcorp.

com/support/Magnetic%20Microspheres/Washing_Method_whitepaper.pdf for more details)

• May be a Bio Tek ELx405 or Tecan Hydroex washer equipped with a washer

manufacturer provided magnet for automated washing

MAGNETIC

SEPERATION

DEVICE

This Quick Guide is not intended to replace the xMAP® Flock Monitor (IBDV, NDV, IBV, REO) Assay Package Insert (document number 89-60000-

00-070 rev. 9). Please refer to the Package Insert for complete background, instructions, warnings and procedure precautions.

• Calculate how much conjugate you will need with the following formula:

Required SA-PE conjugate volume = (0.10 mL) x (number of wells*) + (10%)

• Aliquot this amount into a reagent trough, then cover with foil to protect from light.

• Promptly return SA-PE conjugate to 2-8°C storage.

• Calculate how much conjugate you will need with the following formula:

Required conjugate volume = (0.10 mL) x (number of wells*) + (10%)

• Aliquot this amount into a reagent trough, then cover with foil to protect from light.

• Promptly return detection antibody to 2-8°C storage.

SA-PE

CONJUGATE

DETECTION

ANTIBODY

• WASH Buffer is provided ready-to-use in nal dilution.

WASH

BUFFER

• Calculate the volume of microspheres you will need with the following formula:

Required microsphere volume = (0.05 mL) x (number of wells) + (10%)

• Aliquot the required microsphere volume into another tube or reagent trough, shield

from light, and set aside for use in assay setup.

• Dilute all samples 1:500 using Sample DILUENT. You will need 50 µL of each diluted

sample per well.

• IMPORTANT - Vortex samples before use and after dilution.

• Dilute CONTROLS labeled (-) and (+) 1:500 using Sample DILUENT. You will need 50 µL

of each diluted control per well.

• IMPORTANT - Vortex controls before use and after dilution.

SAMPLES

CONTROLS

MICROSPHERE

MIX

*Consult the product insert for more information

Flock Monitor

IBDV

NDV

IBV

REO

1 2

Quick Guide 1
Plate Layout 2

Résumé du contenu

Page 1 - Quick Guide

Quick Guide 89-30000-00-261 Revision A - Page 1 of 2Sample, Reagent and Plate PreparationBefore setting up the Flock Monitor assay plate, use the foll

Page 2 - Plate Layout

89-30000-00-261 Revision A - Page 2 of 2Protect microspheres, conjugate, and detection antibody from prolonged light exposure throughout entire proce

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Luminex Flock Monitor Quick Guide Manuel d'utilisateur

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