Luminex 200™ System User Manual Lum/nex®
iv PN 89-00002-00-144 Rev. B Luminex 200 System User Manual xMAP Technology
PN 89-00002-00-144 Rev. B 1 - 1 SafetyPlease become familiar with the information in this chapter before using the equipment. Do not perform procedur
Luminex 200 System User Manual xMAP Technology1 - 2 PN 89-00002-00-144 Rev. B Symbols These symbols describe warnings, cautions, and general informati
xMAP Technology SafetyPN 89-00002-00-144 Rev. B 1 - 3switch is not a disconnect means; power cords must be removed from the outlet. Contact Luminex
Luminex 200 System User Manual xMAP Technology1 - 4 PN 89-00002-00-144 Rev. B Access doors must be closed while operating the Luminex 200 analyzer; th
xMAP Technology SafetyPN 89-00002-00-144 Rev. B 1 - 5Decontaminating the Luminex 200 Analyzer for Return ShipmentLuminex Technical Support will give
xMAP Technology SafetyPN 89-00002-00-144 Rev. B 1 - 6
PN 89-00002-00-144 Rev. B 2 - 1The SystemTheory of Operation Luminex 200 technology is based on flow cell fluorometry with Luminex-developed innovat
Luminex 200 System User Manual xMAP Technology2 - 2 PN 89-00002-00-144 Rev. B The optics assembly consists of two lasers. One laser excites the dye mi
xMAP Technology The SystemPN 89-00002-00-144 Rev. B 2 - 3Required Laboratory Reagents• Household bleach• 70% isopropanol or 70% ethanol• Mild deterg
© LUMINEX CORPORATION, 2001-2005. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into any
Luminex 200 System User Manual xMAP Technology2 - 4 PN 89-00002-00-144 Rev. B • Internal sample carry over: < 0.9%• Soluble background fluorescence
xMAP Technology The SystemPN 89-00002-00-144 Rev. B 2 - 5• UL installation category: UL Installation Category II, as defined in Annex J of UL 61010A
Luminex 200 System User Manual xMAP Technology2 - 6 PN 89-00002-00-144 Rev. B • Input voltage range: 100-240 V~ ± 10%, 1.8 Amps, 47-63 Hz• AC inlet fu
xMAP Technology The SystemPN 89-00002-00-144 Rev. B 2 - 7Barcode Labels Use the Code 128 barcode label type when scanning barcode labels into the sy
Luminex 200 System User Manual xMAP Technology2 - 8 PN 89-00002-00-144 Rev. B 1Figure 2-1 Back of the Luminex 200 Analyzer and Luminex XYP Instrument
xMAP Technology The SystemPN 89-00002-00-144 Rev. B 2 - 91. Cheminert Fitting 3. Probe Adjustment Screw2. Probe Holder 4. Luminex XYP I
Luminex 200 System User Manual xMAP Technology2 - 10 PN 89-00002-00-144 Rev. B Air Intake Filter A replaceable air intake filter cleans the air used t
PN 89-00002-00-144 Rev. B 3 - 1Maintenance and CleaningTo ensure accurate test results, properly clean and maintain the Luminex 200 system. Read and
Luminex 200 System User Manual xMAP Technology3 - 2 PN 89-00002-00-144 Rev. B 4. Click Alcohol Flush. A confirmation dialog box opens. Click Eject/Re
xMAP Technology Maintenance and CleaningPN 89-00002-00-144 Rev. B 3 - 35. Loosen the probe adjustment screw on the probe holder one-third to one-hal
Standard Terms and Conditions For Use of ProductBy opening the packaging containing this product ("Product") or by using such Product in any
Luminex 200 System User Manual xMAP Technology3 - 4 PN 89-00002-00-144 Rev. B Figure 3-4 Refill Sheath Fluid Warning (Circled in Photo)Refill the S
xMAP Technology Maintenance and CleaningPN 89-00002-00-144 Rev. B 3 - 5WeeklyVisual Inspection Open all of the Luminex 200 analyzer doors and visual
Luminex 200 System User Manual xMAP Technology3 - 6 PN 89-00002-00-144 Rev. B MonthlyClean Exterior Surfaces To clean exterior surfaces, follow the
xMAP Technology Maintenance and CleaningPN 89-00002-00-144 Rev. B 3 - 7Figure 3-5 Grasping the Tubing4. Remove the filter with one hand, and hold t
Luminex 200 System User Manual xMAP Technology3 - 8 PN 89-00002-00-144 Rev. B Figure 3-6 Removing the Screen3. Replace the filter and replace the s
xMAP Technology Maintenance and CleaningPN 89-00002-00-144 Rev. B 3 - 9Syringe Seal To replace the syringe plunger seal:1. Disconnect the Luminex
Luminex 200 System User Manual xMAP Technology3 - 10 PN 89-00002-00-144 Rev. B 11. Hand-tighten the screw on the syringe arm.12. Plug in the power co
xMAP Technology Maintenance and CleaningPN 89-00002-00-144 Rev. B 3 - 11AnnuallySheath Filter To change the Luminex 200 analyzer sheath filter: 1.
Luminex 200 System User Manual xMAP Technology3 - 12 PN 89-00002-00-144 Rev. B As requiredFuses To replace the fuses in either the Luminex 200 analy
xMAP Technology Maintenance and CleaningPN 89-00002-00-144 Rev. B 3 - 13Replacing the SD System with a Sheath Bottle1. Turn off the SD and disconnec
BUYER HEREBY GRANTS TO LUMINEX A NONEXCLUSIVE, WORLDWIDE, UNRE-STRICTED, ROYALTY-FREE, FULLY PAID-UP LICENSE, WITH THE RIGHT TO GRANT AND AUTHORIZE SU
xMAP Technology Maintenance and CleaningPN 89-00002-00-144 Rev. B 3 - 14• The light above the sample probe on the Luminex 200 and the light next to
Luminex 200 Maintenance Log Month(s): ________________________________________Year:_______________Maintenance LogProcedure: U
PN 89-00002-00-144 Rev. B 4 - 1 TroubleshootingTroubleshooting the Luminex 200 SystemTroubleshooting procedures help users isolate, identify, and rem
Luminex 200 System User Manual xMAP Technology4 - 2 PN 89-00002-00-144 Rev. B Users in Europe can call us at +31-162408333 between 8:30 and 5:30, Cent
xMAP Technology TroubleshootingPN 89-00002-00-144 Rev. B 4 - 3Pressurization Normal air and sheath pressure readings vary between 6-9 psi while the c
Luminex 200 System User Manual xMAP Technology4 - 4 PN 89-00002-00-144 Rev. B Fluid Leaks Fluid leaks can result in poor pressurization and failed sam
xMAP Technology TroubleshootingPN 89-00002-00-144 Rev. B 4 - 5Sample arm is stuck in the down positionThe sample probe height is too low, or the path
Luminex 200 System User Manual xMAP Technology4 - 6 PN 89-00002-00-144 Rev. B Calibration and Control ProblemsSymptom Possible problem SolutionCalibra
xMAP Technology TroubleshootingPN 89-00002-00-144 Rev. B 4 - 7Zero events collected during calibration.There is a problem with fluid levels.Check the
End-User License Agreement (EULA) for Luminex® SoftwareThis Luminex End-User License Agreement (“EULA”) is a legal agreement between you (either an in
Luminex 200 System User Manual xMAP Technology4 - 8 PN 89-00002-00-144 Rev. B Acquisition ProblemsThere is air in the system. Verify sample probe heig
xMAP Technology TroubleshootingPN 89-00002-00-144 Rev. B 4 - 9Bead Detail IrregularitiesUse these tools to assist in diagnosing system and kit-relate
Luminex 200 System User Manual xMAP Technology4 - 10 PN 89-00002-00-144 Rev. B A normal bead detail display is shown below. It depicts a tight bead po
xMAP Technology TroubleshootingPN 89-00002-00-144 Rev. B 4 - 11xMAP microspheres hit the lower right of the region.You may be using photobleached xMA
Luminex 200 System User Manual xMAP Technology4 - 12 PN 89-00002-00-144 Rev. B Error States The following error states display on the Status Bar on th
xMAP Technology TroubleshootingPN 89-00002-00-144 Rev. B 4 - 13Low Voltage Detected Possible laser failure. Turn off the analyzer, XYP, and PC, then
Luminex 200 System User Manual xMAP Technology4 - 14 PN 89-00002-00-144 Rev. B Sample Error MessagesSample error messages occur when there is a proble
xMAP Technology TroubleshootingPN 89-00002-00-144 Rev. B 4 - 15Sample High or Low The results do not fall within the highest and lowest standards.Ver
Luminex 200 System User Manual xMAP Technology4 - 16 PN 89-00002-00-144 Rev. B Luminex SD ProblemsIf the empty sheath fluid container is not replaced
xMAP Technology TroubleshootingPN 89-00002-00-144 Rev. B 4 - 17Malfunction If the alarm sounds even though the bulk sheath container has fluid and t
5. EXPORT RESTRICTIONS. You agree that you will not export or re-export the SOFTWARE PRODUCT to any country, person, entity, or end-user subject to U.
Luminex 200 System User Manual xMAP Technology4 - 18 PN 89-00002-00-144 Rev. B
PN 89-00002-00-144 Rev. B 5 - 1Product NumbersHardware5Product Description Customer NumberRear Air Filter CN-0001-01Bottom Air Filter CN-0002-01Air
Luminex 200 System User Manual xMAP Technology5 - 2 PN 89-00002-00-144 Rev. B SoftwarexMAP ReagentsPower Cord, USAPower Cord, AustraliaPower Cord, Br
xMAP Technology Product NumbersPN 89-00002-00-144 Rev. B 5 - 3Microspheres, LX100, CL1 CL2 Calibration (xMAP Classification Calibrator)L100-CAL1Micr
Luminex 200 System User Manual xMAP Technology5 - 4 PN 89-00002-00-144 Rev. B
PN 89-00002-00-144 Rev. B Safety 1-1Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1Warnings and
ii PN 89-00002-00-144 Rev. B Luminex 200 System User Manual xMAP TechnologyFluidics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
PN 89-00002-00-144 Rev. B iiixMAP Technology ContentsSample Error Messages. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-14Lumin
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