Luminex xMAP Antibody Coupling Kit Manuel d'utilisateur télécharger pdf (Page 15)

Page 11 of 20

16. Wash the microspheres.

A. Place the reaction tube with microspheres into the magnetic separator for 1-2

minutes.

B. With the tube still positioned in the mag

netic separator, remove the supernatant

with a transfer pipette.

C. Add 500 μL

of Activation Buffer into the reaction tube.

D. Vortex the reaction tube for 10 seconds

and then sonicate for 10 seconds to

disperse the microspheres.

17. Repeat Step 16 twice, for a total of three washes.

18. Place the reaction tube with microspheres into

the magnetic separator for 1-2

minutes and, with the tube still positioned in the magnetic separator, remove the

supernatant with a transfer pipette.

19. Calculate volume of antibody

to be used in the reaction.

• Typically, a range of 2-5 μg of antibody per 1x10

microspheres is a good

starting point, if performing the coupling reaction for the first time.

Example Calculation:

If coupling 5 million microspheres, with an a

ntibody at a concentration of 5 mg/mL,

and using the suggested starting point of 5 μg of antibody per 1x10

micro-

spheres:

1 2 ... 10 11 12 13 14 15 16 17 18 19 20 ... 23 24

Pas de commentaire

Luminex xMAP Antibody Coupling Kit Manuel d'utilisateur Page 15