Luminex xMAP Antibody Coupling Kit Manuel d'utilisateur télécharger pdf (Page 21)

Page 17 of 20

Troubleshooting

This troubleshooting guide may be helpful in solving problems that may arise. For more

information, call Technical Support toll free at 1-877-785-BEAD (2323). Outside of U.S.

and Canada, call +1 512-381-4397. Send e-mail inquiries to: [email protected]

Problem Possible Cause(s) Recommendations

Low Bead

Count

Uncoupled

microspheres tend to

stick to the inner

surfaces of some

tubes

Make sure to use tubes supplied in this

kit only.

Microspheres were

lost during washes

Use the recommended magnetic

separator to perform all washes. See

Recommended Material list.

Use the transfer pipette to remove all

supernatant during washes. The

microsphere pellets are loose and

using a pipettor could disturb the bead

pellet.

Incorrect probe height

adjustment

Adjust probe height according to the

Luminex

200

™

, FLEXMAP 3D

MAGPIX

user manual.

Incorrect protocol set-

up on the Luminex

instrument

Make sure correct microsphere

regions are selected based on your

particular custom designed assay.

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Luminex xMAP Antibody Coupling Kit Manuel d'utilisateur Page 21