Luminex xMAP Antibody Coupling Kit Manuel d'utilisateur télécharger pdf (Page 20)

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8. Mix the reactions gently by pipetting up an

d down several times with a pipettor.

9. Cover the plate and incubate for 30 minutes at room

temperature on a plate shaker.

10. Clip the plate in place on the Luminex

Magnetic Plate Separator and rapidly and

forcefully invert over a biohazard receptacle to evacuate the liquid from the wells.

NOTE: For information on the MagPlex Manual Wash Method, please visit:

http://www.luminexcorp.com/Products/Re

agentsMicrospheres/MAGPLEX-

MICROSPHERES.

11. Wash each well with 100 μL o

f PBS-1% BSA by gently pipetting up and down

several times with a pipettor, and remove the liquid by using the procedure

described in the previous step.

12. Repeat the previous wash step once.

13. Resuspend the microspheres in 100 μL of

PBS-1% BSA by gently pipetting up and

down several times with a pipettor.

14. Analyze 50-75 μL o

n the Luminex analyzer according to the system manual. An

example of typical results is shown below.

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Luminex xMAP Antibody Coupling Kit Manuel d'utilisateur Page 20