Luminex BioPlex XYP, LX200 User Manual (supplied by BioRad Manuel d'utilisateur télécharger pdf (Page 25)

Fig. 17. Sample needle assembly.

11. By holding onto the needle height adjustment thumbscrew on the needle arm, manually

move the needle so that it just touches the bottom of the needle adjustment well of the

MCV plate. Move the needle up and down gently a couple of times to verify that the needle

is barely touching the bottom of the well.

12. Tighten the needle height adjustment thumbscrew so that it is no longer possible to manually

move the needle up and down. Take care to ensure that the needle does not move while you

are tightening the screw. Do not overtighten.

13. In the Adjust Needle window, click on the Up/Down button to move the needle up and

down. Look inside the microplate platform at the MCV plate. The needle should just touch

the MCV plate at the bottom of the cutout (use flashlight for better viewing). Readjust the

needle height if necessary.

14. When the needle is adjusted properly, click the Eject button across the top of the title bar.

15. Remove the MCV plate from the microplate platform.

16. Click on the Close button in the Adjust Needle window.

17. Perform a Wash Between Plates step to remove any air introduced into the lines.

3.9 Initial System Priming

This procedure is to be performed only during the initial installation of the array reader.

1. Fill the maintenance, calibration, and validation (MCV) plate (Figure 18) with deionized

water and 70% isopropyl alcohol in the appropriate wells.

Fig. 18. MCV plate IV.

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Needle height adjustment

thumbscrew

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Luminex BioPlex XYP, LX200 User Manual (supplied by BioRad Manuel d'utilisateur Page 25