Luminex IS Version 2.3 Manuel d'utilisateur télécharger pdf (Page 105)

PN 89-00002-00-110 Rev. B 95

xMAP Technology Luminex xMAP microsphere set

Luminex xMAP

microsphere set

Luminex multi-analyte microspheres containing a unique mixture of

two distinctly colored fluorochromes to distinguish them from other

multi-analyte microspheres.

laser Light Amplification by Stimulated Emission of Radiation (laser).

This highly purified source of light is an efficient way to excite

fluorochrome electrons.

microparticle A solid substance with a diameter in the micrometer range. Often

used as a synonym for a microsphere.

microspheres Polystyrene spheres with a diameter in the micrometer range. Also

called beads.

multi-analyte Several assays or tests performed simultaneously in the same

reaction container.

multi-batch A set of batches to be processed consecutively.

photobleaching The process in which light absorption converts the fluorochromes

inside the beads into different fluorescent or nonfluorescent

compounds. Photobleaching prevents beads from being properly

classified.

PMT Photomultiplier tube, measures the excitation emission intensity of

the reporter dye bound to the surface of the xMAP microspheres.

product A group of standards and controls that you use to create a template.

Products are analogous to “reagent kits”. They are used in the

template’s definition for the multi-analyte assays that will be

performed.

Quali A value that determines a cutoff or threshold. This in, conjunction

with ranges using the Lum Qual Formula, Adv Qual Formula, or an

edited range specific for your assay, helps to determine qualitative

results for unknown samples.

qualitative Pertaining to calculations that determine the absence or presence of

an analyte.

quantitative Pertaining to calculations that determine the precise numerical

measurement of an analyte.

reporter A molecule (or combination of molecules) with a specific range of

excitation and emission wavelengths that is used to identify or

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Luminex IS Version 2.3 Manuel d'utilisateur Page 105