Luminex BioPlex XYP, LX200 User Manual (supplied by BioRad Manuel d'utilisateur télécharger pdf (Page 39)

Bio-Plex Manager has Most likely:

detected a change in

sheath pressure. Sheath reservoir cap not Tighten sheath cap. Click OK.

on securely Message should disappear within 2

min.

Sheath bottle lines are not Make sure that all hoses are connected

connected properly to the appropriate ports, and that they

clicked into place.

Less likely:

Sheath fluid level above Adjust sheath fluid level so that sheath

the AIR port on the sheath fluid is below the AIR port of sheath

container bottle.

Sheath and waste bottle Make sure that the blue-ringed cap is

caps are reversed on the sheath bottle and the orange-

ringed cap is on the waste.

Sheath bottle has a leak Try new sheath bottle. Call technical

service for further assistance.

HTF unit not turned on Turn on HTF unit and ensure that

message disappears.

Air compressor not Listen for air pump to turn on when

working Warm Up is selected. Contact

Technical Support for further assistance.

Bio-Plex Manager has Room temperature has Calibrate array reader.

detected a change in the changed

temperature of the array

reader. Please calibrate

before running on assay to

ensure accurate results.

The calibration was Calibration procedure Make sure Cal1 beads and Cal2

unsuccessful. Please repeat failed beads are placed in the appropriate

calibration. If calibration wells (Cal1 in red well and Cal2 in

fails a second time, consult green well). Repeat calibration. Make

Troubleshooting Guide. sure you are using a clean MCV plate.

The calibration was Calibration procedure Run Unclog procedure, then repeat

unsuccessful. Bio-Plex failed due to low bead calibration. If problem persists, contact

Manager has detected a number Technical Support. See “low bead

problem with low bead number” in troubleshooting guide.

number. Please repeat

calibration.

Optical Validation Problem with optical Recalibrate the array reader and then

Procedure shows value(s) component of array repeat validation procedure. If values

outside of acceptable reader are still out of range, contact Technical

range(s). Support.

Reporter Validation Problem with optical Recalibrate the array reader and then

Procedure shows value(s) component of reader repeat validation procedure. If values

outside of acceptable are still out of range, contact Technical

range(s). Support.

Classify Validation Problem with calibration Recalibrate the array reader and then

Procedure shows value(s) or optical component of repeat validation procedure. If values

outside of acceptable reader are still out of range, contact Technical

range(s). Support.

www.bio-rad.com/bioplex/

Message/Problem Causes Solution

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Luminex BioPlex XYP, LX200 User Manual (supplied by BioRad Manuel d'utilisateur Page 39

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