Luminex FLEXMAP 3D Hardware User Manual Manuel d'utilisateur télécharger pdf (Page 53)

Symptom Possible cause Solution

There is air in the instrument. The

waste line was moved during

instrument operation, resulting in

an unstable flow rate.

Perform an automatic sample

probe height adjustment. Use the

software to run a prime command

three times, an alcohol flush

command twice, then a wash

command three time with distilled

water.

The waste line was moved during

instrument operation, resulting in

an unstable flow rate.

Stabilize the waste line during

instrument operation. See the

section titled “Maintaining Fluids”.

There is a problem internal to the

instrument.

Review the log of calibration

reports. Check for dramatic

changes in temperature, sheath

pressure, or voltage. If any of

these are present, contact

Technical Support.

No events are collected

during calibration.

There is a problem with fluid

levels.

Check the sheath and waste fluid

levels. Verify that tubing for both

bottles is tightly connected to the

instrument. Check that the waste

fluid container cap is vented.

The sample probe is clogged. See the section titled "Clogs".

The Cheminert fitting is loose. Tighten the Cheminert fitting.

Verification Problems

TABLE 7.

Verification Problems

Symptom Possible cause Solution

The instrument fails

verification.

The verification microspheres are

not fully suspended.

Vortex the verification vials to

resuspend the microspheres.

The wrong lot number or target

values are entered in the

appropriate page of the software

UI.

Check the lot number and target

values to make sure they are

correct.

The instrument verification

microspheres are in the wrong

well.

Verify that verification

microspheres are in the correct

well.

There are not enough verification

microspheres in the well.

Add at least five drops of

verification microspheres to the

well. For accurate drop volume,

hold the vial upside down at a 90

degree angle to the microtiter plate

while dispensing them.

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Luminex FLEXMAP 3D Hardware User Manual Manuel d'utilisateur Page 53

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