Luminex FLEXMAP 3D Hardware User Manual Manuel d'utilisateur télécharger pdf (Page 55)

Symptom Possible cause Solution

The wrong lot number or target

values are entered in the

appropriate page of the software

UI.

Check the lot number and target

values to make sure they are

correct.

Air is present in the instrument. Verify the sample probe height.

Use the software to run a prime

command three times, an alcohol

flush command twice, then a wash

command three times with distilled

water.

The acquisition volume is set too

high.

Set the acquisition volume to at

least 25 μL less than the actual

volume in your wells. This enables

the probe to acquire sample more

efficiently with less chance of

incorporating air in the sample.

The xMAP microspheres are not

fully suspended.

Gently vortex the microtiter plate

or resuspend beads with a

multichannel pipettor to ensure

that the microspheres are present

in the solution.

The microspheres are

photobleached.

Replace the microspheres with

unphotobleached microspheres.

There are an insufficient number

of beads in the sample.

Ensure that there are 2000 to

5000 beads per bead set per well.

The sample is too concentrated. Dilute concentrated biological

fluids, such as serum or plasma, at

least 1:5.

Bead Detail Irregularities

Use these tools to assist in diagnosing system and kit-related problems:

• instrument calibrators

• instrument verifiers

• assay standards

• assay controls

• error messages

Review calibration/control trend reports routinely to detect trends.

Use instrument xMAP verification microspheres to check the success of the instrument

calibration and to troubleshoot. If there is a problem with kit results, xMAP verifiers can help

determine if the problem is related to the instrument. If calibration and verification are

successful, contact the kit manufacturer.

The Bead Detail Irregularities table displays irregular groupings of beads. Compare the

displays to the following bead detail of a normal bead grouping - a tight bead population

within a white region.

FLEXMAP 3D

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Luminex FLEXMAP 3D Hardware User Manual Manuel d'utilisateur Page 55

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