Luminex IS Version 2.3 Manuel d'utilisateur télécharger pdf (Page 50)

Luminex IS Software Manual for Version 2.3 xMAP Technology

40 PN 89-00002-00-110 Rev. B

Startup Procedures

Warm Up the

System

Warm up the system to prepare the optics prior to sample acquisition.

The system automatically begins warming up when you turn power

on. This procedure takes approximately thirty minutes.

Caution: Failure to properly warm up the system will effect assay

results and system performance.

After four hours of inactivity, the status bar appears red and indicates

that the lasers are off. You need to warm up the system again by

manually initiating warmup.

 To warm up the system:

Click Warmup, then click OK. The command list on the Run

Batch tab indicates that the system lasers are running. The

Device Activity box on the Status Bar indicates that the system is

warming. The Laser Status section on the Status Bar is yellow as

it counts down from 1800 seconds. Upon completion, the Laser

Status bar turns green and displays Warmed Up.

Prime the System Prime your system as part of the daily startup routine and as

necessary to remove air from the system’s fluidic pathways after:

• refilling the sheath container

• removing and replacing the sheath container

• observing air in the tubing

• changing the sheath fluid filter

• changing the syringe seal

When priming, the system draws Luminex xMAP sheath fluid from

the sheath fluid container and sends it directly out to waste, and takes

approximately one minute. You do not supply solution in a plate.

 To prime the system:

Click Prime, then click OK to confirm that you want to prime

the system. The status bar indicates that the prime command is

processing.

Backflush the

System

Backflush the system:

• to remove obstructions from the cuvette

• if fluid does not flow through the waste tubing during prime

cycles or during sample acquisition

• if fluid drips from the sample probe during priming and forms

puddles of fluid on the plate

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Luminex IS Version 2.3 Manuel d'utilisateur Page 50